摘要
目的对比研究氛吡格雷抑制血小板聚集的两种方法：Verifynow抗血小板功能检测系统与血栓弹力图实验。方法①随机选取2011年3月～7月在解放军总医院住院及门诊的急性冠状动脉综合征（ACS）患者48例（所有患者对本实验均知情同意），男性35例，女性13例，年龄60-90岁，其用药方案是首先给患者300mg的负荷量，继之以75mg／d维持量治疗。②选择在治疗的第三天服用氯吡格雷75mg／d后2h抽血取样。③采用Verifynow抗血小板监测系统检测氯吡格雷药物抑制率1NHI（％），血栓弹力图实验检测氧吡格雷药物的ADP诱导血小板聚集抑制率（％），两指标进行相关分析、回归分析。结果48例观察对象均进入结果分析，Verifynow的INHI（％）主要集中在0％～20％之间，犬于20％的有10例，占总数的20．8％，其结果的变异系数（CV）为0．74％；血栓弹力图的ADP诱导血小板聚集抑制率结果主要集中在10Voo～60％，小于10％的对象2例，占总数的4．17％，大于60％的对象6例，占总数的12．5％，其结果的变异系数（CV）为：0．54％；两者呈正相关（r相关系数=0．4806，P-0．00054〈0．01）；回归方程：Y=0．267X＋4。577[Verifynow的1NHI（％）为因变量Y；血栓弹力图的ADP诱导血小板聚集抑制率为自变量X]。结论口服氯吡格雷后，Verifynow抗血小板监测系统的INHI（％）与血栓弹力图的ADP诱导血小板聚集抑制率有较好的一致性，其操作更为简便、费用更为低廉，可以作为临床氯吡格雷药物抗血小板功能监测的新方法。
Abstract：Objective To comparatively study the difference between the platelet aggregation experiment tested by Verifynow antiplatelet function test system and by Thrombelastography analyzer. Methods①Totally 48 acdte coronary syndrome pa- tients including male 35 and female 13,who were in hospital or the outpatients of tile PLA General Hospital from March to June 2011 and whose ages ranged from 60 to 90 years old,were selected randomly. They took 300mg clopidogel at begin- ning, after that, they were treated by 75mg clopidogrel. ②To draw the blood samples after the observed patients took 75 rag/ d clopidogrd two hours in the third day.③The INHI（%） was tested by the Verify＇now antiplatelet function test system and the inhibition ratio was examined with Thrombelastography analyzer, the two index carried out the correlation analysis and regression analysis. Resulls All the 48 observed patients entered the result analysis. The INHI（%） tested by the Verifynow antiplatelet function test system from the 48 patients mainly concentrated on the range from 0 to 20%,there were 10 observ- ers whose INHI （ %） were more than 20 %, which accounted for the total number of 20.8 %, the coefficient of variation（CV） of all the results was 0. 240 %. The inhibition ratio of the platelets tested by the Thrombelastography analyzer mainly concen- trated on the range from 10 % to 60%, there were 2 observers whose results were 10%, which accounted for the total num- ber of 4.17 % ,there were 6 observers whose results were more than 600//00 ,which accounted for the total number of 12. 5 %, the coefficient of variation（CV） was 0. 54. %. The two index submit positive correlation（ r related coefficient= 0. 480 6, P= 0. 000 54〈0.01） ,the linear regression equation：Y=0. 267X＋4. 577[the INHI（ %） tested by Verifynow antiplatelet func- tion test system was the dependent variable Y, the inhibition ratio of the platelets examined with the Thrombelastography analyzer was the independent variable X]. Conclusion After the observed patients took the clopidogrel,the correlation of the INHI（ %） tested by the Verifynow antiplatelet function test and the inhibition ratio of the platelets tested by the Throm- belastography analyzer was good, furthermore, the expense of the Verifynow antiplatelet function test system was more low-er and its operation was simpler than the Thrombelastography analyzer.
出处
《现代检验医学杂志》
CAS
2012年第2期114-117,共4页
Journal of Modern Laboratory Medicine
