摘要
丝瓜Luffa cylindrica (L.)Roem。与天花粉同科,均系葫芦科植物。鉴于天花粉蛋白和四季豆蛋白等有抗生育作用,我们选用丝瓜子蛋白对小鼠进行了抗早孕作用研究。
The crude protein was extracted from the seeds of Luffa cylindrica (L.) Rocm. It was shown that crude protein terminated early prcgnancy in mice by intrapcriotoncal injection on day 7 of gestation. The crude protein was further prifide on hydroxylapatite column (6×5cm) equlibrated with 0.001 mol/L phosphate buffer pH 6.8, and elution was carried out with the same buffcr (0.001, 0.05, 0.1 and 0.2 tool/L, respectively). The flow rate was 46 ml/h, and four fractions were obtained. One of these fractions, Peak Ⅱ, eluted by 0.05 tool / L pH 6.8 phosphate buffer, showed a single band on agar gel elcctrophorcsis. In early pregnant mice, terminating pregnancy rate by the main componcnt (Peak Ⅱ) was 100% at dose 3.3 mg / kg body weight by intraperitoneal injection.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1990年第2期115-116,共2页
Journal of China Pharmaceutical University
关键词
丝瓜
丝瓜子
蛋白
抗早孕
Secds of Luffa cylindrica (L.) Roem.
Protein
Terminative early pregnancy
