摘要
建立了以离子液体([HMIm]BF4)为萃取剂,HPLC检测水产品中残留司帕沙星的方法,采用反相SpherisorbC18色谱柱(250mm×4mmi.d.,5μm),流动相为甲醇,流速:0.5mL/min,检测波长:298nm。在0.06～0.18mg/mL浓度范围内,方法线性良好(R2=0.9988),回收率为88.7%～93.0%,日内和日间的RSD介于2.1%～3.7%。方法准确可靠,重现性好,灵敏度高,适用于水产品中残留药物的检测。
Determination of sparfloxacin in the aquatic products using [HMIm] BF4 as extraction agent coupled with HPLC was investigated.The column configuration consisted of Spherisorb C18 column（250 mm×4 mm i.d.,5 μm particle size）.The mobile phase was methanol at a flow rate 0.5 mL/min,the UV detector was set at 298 nm.calibration curve was linear over the concentration ranges tested with 0.06～0.18 mg/mL（R2=0.9988）.The precision（RSD%） and accuracy were 2.1～3.7 and 88.7%～93.0%,respectively.The method was validated for linearity,precision and intra-and inter-day variation,and was suitable to the determination of drug residues in aquatic products.
出处
《食品科技》
CAS
北大核心
2012年第5期303-306,共4页
Food Science and Technology
基金
浙江省自然科学基金项目(Z3100533
Y2111223)
浙江省科技厅公益计划项目(2011C22073)
浙江省新苗计划项目(2010R411012)
关键词
离子液体
高效液相色谱
水产品
司帕沙星
ionic liquid
high performance liquid chromatography
aquatic products
sparfloxacin
