摘要
以1-苄基-3-乙基苯并咪唑六氟磷酸盐作为萃取试剂,1-丁基-3-甲基咪唑六氟磷酸盐作为辅助溶剂及载体,按照1∶9(V/V)混合,得到混合离子液体。利用混合离子液体液-液分散萃取养殖水及水产品中的孔雀石绿及结晶紫,并优化萃取条件。通过高效液相色谱-荧光检测器检测孔雀石绿及结晶紫的含量。结果表明,选择混合型离子液体进行吸附,以乙腈为分散剂,采用液-液分散萃取,在pH值为4~8的环境下对目标物进行萃取,效果良好。孔雀石绿和结晶紫回收率分别为90.16%~93.73%和90.25%~93.88%,相对标准偏差(RSD)分别为1.42%和1.63%。表明所建立的样品前处理方法及检测方法准确度高,可用于孔雀石绿及结晶紫的检测。
With 1-benzyl-3-ethyl benzimidazole hexafluorophosphate as extraction reagent, 1-butyl-3-methylimidazolium hexafluorophosphate as auxiliary solvent and carrier, mixing with 1∶9 （V/V）, the mixed ionic liquid was obtained. The extraction conditions of the malachite green and crystal violet in aquaculture water and aquatic products were optimized by mixed ion liquid liquid-liquid dispersion extraction. The contents of the malachite green and crystal violet were detected by high performance liquid chromatography-fluorescence detector. The results showed that selecting the mixed ion liquid for adsorption, with acetonitrile as a dispersant, the target objects were extracted in pH 4-8 by liquid-liquid dispersion extraction, and the extraction effect was good. The recovery rate of malachite green and crystal violet were 90.16%-93.73% and 90.25%-93.88%, respectively. The relative standard deviation （RSD） were 1.42% and 1.63%, respectively, which indicated that the established sample preparation method and detection method had high accuracy, and could be used for the detection of malachite green and crystal violet.
出处
《中国酿造》
CAS
北大核心
2017年第12期163-167,共5页
China Brewing
基金
甘肃省科技计划资助(1606RTSA337
1309RTSA025
1605JTCA011)
甘肃省公共安全检验检测服务平台项目
