摘要
以甘蔗品种＂粤糖55号＂为材料,对其离体快繁技术进行了研究。结果表明,用带腋芽的甘蔗茎段作为外植体,经0.1%HgCl2消毒7min后,接种到MS＋6-BA 1.0 mg/L＋NAA 0.2 mg/L的培养基上进行启动培养,能够诱导丛生芽。丛生芽继代和生根最优的培养基分别为MS＋6-BA 1.0 mg/L和MS＋NAA 2.0mg/L。甘蔗组培苗生根炼苗后,移到木糠中进行假植,成活率为90%~95%。
The rapid micropropagation technique of sugarcane variety YT55 was studied. Lateral buds as explants source were disinfected by 0.1% solution of mercuric chloride for 7 minutes,then to plant in MS medium with 1.0 mg/L 6-BA and 0.2 mg/L NAA for shoot inducing. The results showed that lots of plantlets were induced. MS was the optimum medium with 1.0 mg/L 6-BA and the medium with 2.0 mg/L NAA for rapid micropropagation and rooting response,respectively. The survival rate was between 90% and 95% when the sugarcane plantlets were transplanted in the medium of wood chaff.
出处
《中国糖料》
2012年第2期63-65,共3页
Sugar Crops of China
基金
2009年广西农业综合开发项目
关键词
甘蔗
粤糖55号
腋芽
离体快繁
sugarcane
YT55
lateral buds
rapid micropropagation
