摘要
以三个甘蔗品种的幼叶和叶鞘为外值体，经愈伤组织途径诱导出体细胞胚，并通过低温处理和渗透压调节法使胚状体发育达到部分同步优。同自配人工胚乳包埋手工剥离出的成熟胚状体，得到直径约5mm的粒状人工种子，其在无菌条件下的发芽率为93.8％，转株率为81．4％。
The somatic emhryos of three sugarcane varietles were induced fomatlon through calll path-way with leaf blade and sheath as explant. The identlty of embryoid development could be improvedpartly by way of Iower temPreiure treatment of embryonic calli and adjtlst osmosls pressure of media.To emhed niature ernbryoids with artlficial endosprm had obtained the granular artificial seeds and itsdlameter was flhollt 5rnm, the germination rate and plantlet formation rate were 93. 8 y6and 81. 4 T6respecrively unde: sterile condition
出处
《甘蔗糖业》
1995年第1期8-13,共6页
Sugarcane and Canesugar
基金
四川省科委应用基础研究基金
关键词
甘蔗
胚状体
人工种子
sugarcane
embryoid
artificial seeds
