摘要
目的:观察大鼠急性脊髓损伤后半胱天冬酶8在组织与血浆中的表达和含量的变化关系及意义。方法:W istar大鼠共84只,随机分成7组,每组12只。其中,6组制作成急性脊髓损伤模型,分为损伤后0h、6h、12h、24h、48h、72h组。另1组作为对照组。应用免疫组化法和酶联免疫法测定损伤后,各个时段脊髓组织和血浆中的caspase-8值。结果:急性脊髓损伤后,组织与血浆内即刻出现caspase-8的阳性表达,伤后24-48h,组织及血浆中caspase-8表达达到峰值,至伤后72h开始降低但仍高于正常对照组。结论:脊髓损伤早期,组织与血浆中的caspase-8表达明显增加,且在时间上具有相应的变化关系,有望为脊髓损伤的临床干预时机的选择,带来一定的帮助。
Objective：To observe relationship and significance of changes in the expression and the level of Caspase-8 in the tissue and blood plasma after rat spinal cord was acutely injured.Methods：Eighty-four Wistar rats were randomly divided into seven groups with 12 in each group,and one group was used as control group and the 6 others were prepared into acute spinal cord injury models,as 0h,6h,12h,24h,48h and 72h injury groups.The caspase-8 value in the tissue and blood plasma at different time points was detected after spinal cord injury by applying immunohistochemical method and ELISA.Results：Soon after acute spinal cord injury,the tissue and blood plasma Caspase-8 showed the positive expression,at 24h-48h,the Caspase-8 expression in the tissue and blood plasma reached a peak,at and 72h after injury began to decrease but still higher than that of the normal control group.Conclusion：At the early stage of the spinal cord injury,the Caspase-8 expression in the tissue and blood plasma is significantly increased,with a corresponding relation to time,which will help the selection of the opportunity of the clinic intervention in acute spinal cord injury .
出处
《黑龙江医药科学》
2012年第2期5-6,共2页
Heilongjiang Medicine and Pharmacy
