摘要
以黄顶菊新鲜植株为材料,用蒸馏水浸提活性物质,得到0.1、0.05和0.01g/mL三种不同干物质含量的黄顶菊水提液。以蒸馏水为对照,利用不同含量的黄顶菊水提液处理无土栽培的玉米幼苗,每4d测定幼苗叶片中MDA(丙二醛)、POD(过氧化物酶)、SOD(超氧化歧化酶)、CAT(过氧化氢酶)和CHL(叶绿素)含量。结果表明,黄顶菊水提液处理对玉米幼苗株高产生了明显的抑制作用,且每个处理与对照相比差异达显著水平(P<0.05)。黄顶菊水提液中含量越高,抑制作用越显著。幼苗叶片中MDA含量、POD、SOD、CAT活性均高于对照,而CHL含量低于对照。研究还发现,不同含量处理和不同处理时间对玉米幼苗MDA含量、抗氧化酶和CHL含量的影响之间存在极显著的交互作用。结果预示黄顶菊水提液能够抑制幼苗生长,含量越大,作用效果越明显,可为进一步研究黄顶菊总体化感提供参考。
The maize seedlings grown in soilless media were treated with 3 concentrations （0. 1,0.05 and 0.01g/mL） of aqueous extract of aerial part of Flaveria bidentis, and distilled water as control. Performances of MDA, POD, SOD, CAT and CHL of the maize leaves were measured and evaluated every four days. Results showed that 3 treatments had negative effects on the growth of maize. The negative effects were enhanced with the increase of extracts concentration. The level of MDA contents, POD, SOD and CAT activity of maize seedling leaves were significantly higher than con- trol, but the content of CHL was obviously less than the control. Moreover, a significant interaction existed between the concentrations of F. bidentis aqueous extracts and the treated time. It turned out that the extracts of F. bidentis could in- hibit the seedling growth. Thus, the results provide evidence for the further research of F. bidentis.
出处
《中国农业气象》
CSCD
北大核心
2012年第2期226-231,共6页
Chinese Journal of Agrometeorology
基金
公益性行业科研专项"新外来入侵植物黄顶菊防控技术体系研究"(200803022)
公益性行业科研专项"入侵植物综合防控技术研究与示范推广"(201103027)
