摘要
取体外培养生长良好脂肪细胞,培养介质中分别添加0、10、20、40、80、160mg/L地塞米松,0、0.5、1.0、1.5、2.0、2.5mmol/L油酸,0、10、20、30、40、50mg/L乳酸,培养24h后提取总RNA,每个处理3个重复,分别采用荧光定量PCR法检测地塞米松、油酸、乳酸对脂肪细胞HSL mRNA丰度的影响。结果表明油酸、乳酸抑制脂肪细胞内HSL mRNA表达,抑制作用随浓度增加而增强;地塞米松促进脂肪细胞内HSL mRNA表达,是剂量依赖性。
A test was designed to investigate whether hexadecadrol,oleic acid and lactic acid affect the expression of HSL mRNA in adipose cells from a newborn Holstein calf.Hexadecadrol,oleic acid and lactic acid were added to the media with 0,10,20,40、80,160 mg/L and 0,0.5,1.0,1.5,2.0,2.5 mmol/L and 0,10,20,30,40,50 mg/L,respectively.Abundance of HSL mRNA in adipose cells was determined by fluorescent quantitative RT-PCR.The results showed that the expression of HSL mRNA was suppressed in adipose cells treated with oleic acid and lactic acid,with dose dependent respectively.the expression of HSL mRNA was promoted by hexadecadrol with dose dependent.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第5期765-770,共6页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(3023062)
教育部
长江学者和创新团队发展计划资助项目(IRT0848)
四川农业大学优秀学士论文培育计划资助项目
