摘要
为阐明胰岛素样生长因子Ⅰ(IGF-Ⅰ)、胰高血糖素样肽Ⅰ(GLP-Ⅰ)在乳牛脂肪代谢过程中的调控作用,应用荧光定量PCR法检测IGF-Ⅰ、GLP-Ⅰ对体外培养的脂肪细胞激素敏感脂肪酶(HSL)mRNA丰度的影响。结果显示,随着IGF-Ⅰ、GLP-Ⅰ添加浓度的升高,脂肪细胞HSL mRNA表达逐渐下降。结果表明,IGF-Ⅰ、GLP-Ⅰ直接调控脂肪细胞HSL mRNA的表达。
In order to investigate the regulation roles of IGF-Ⅰand GLP-Ⅰin the lipometabolism of dairy cows,the effects of them in bovine were observed by the expression level of HSL mRNA of cultured neonatal calf lipocytes using fluorescent quantitative RT-PCR.The expression level of HSL mRNA decreased following with the increasing concentrations of IGF-Ⅰand GLP-Ⅰ in the media.These results indicated that the IGF-Ⅰand GLP-Ⅰcould regulate the mRNA expressions of cultured adipose cells HSL directly.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2011年第12期1266-1270,共5页
Chinese Veterinary Science
基金
国家自然科学基金资助项目(3023062)
四川省教育厅重点项目(10ZA048)
四川农业大学优秀学士毕业论文培育计划项目
