摘要
采用醋酸溶液和乙醇提取了6支马鹿茸的主杠尖部、中部、根部3个部位的多肽,进行了IGF-1的SDS-PAGE和Western blot分析,采用RIA法测定了马鹿茸酸粗提物及醇提多肽的IGF-1含量。结果表明,酸粗提物中IGF-1存在游离和与结合蛋白结合两种形式,醇提物中只存在分子量为7.5 ku的游离IGF-1。马鹿茸不同部位酸粗提物和醇提物之间IGF-1含量经RIA法检测表明,主杠尖部、中部、根部3个部位IGF-1含量差异显著,其中尖部IGF-1含量分别比中部和根部高43.10%(P<0.01)、284.83%(P<0.01),中部比根部高168.93%(P<0.01);经醇提后,各部位之间IGF-1含量差异显著,其中尖部IGF-1含量分别比中部和根部高42.33%(P<0.05)、164.04%(P<0.01),中部比根部高85.51%(P<0.05)。该试验为马鹿茸的分段利用提供了内在质量上的依据。
In this study , acid extract and alcohol extract of six cervus elaphus linnaeus were obtained. Insulin-like growth factor I（IGF- 1）was identified by SDS-PAGE and Western blot. IGF- 1 contents in different segments （top,middle ＆ basis,three segments）were detected with RIA. The results of SDS - PAGE and Western blot showed that IGF- 1 was extracted with acid and alcohol. IGF- 1 contents of the acid extract in top was higher 43.10%（ P 〈 0.01）and 284.83% （P 〈 0.01）than in middle and basis respectively, was higher 42.33% （ P 〈 0.05） and 164.04% （ P 〈 0.01 ） for alcohol extract polypeptide. IGF-1 contents of the acid extract in middle was higher 168.93 % （P 〈 0.01） than in basis, was higher 85.51 % （ P 〈 0.05） for alcohol extract polypeptide. This test offered an internal quality index in subsection utilization of cervus elaphus linnaeus.
出处
《经济动物学报》
CAS
2005年第4期201-203,共3页
Journal of Economic Animal
基金
本溪天山鹿业新技术有限公司合作项目
关键词
马鹿茸
多肽
胰岛素样生长因子1
比较分析
Cervus elaphus linnaeus
polypeptide
IGF - 1
comparative analysis
