摘要
观察小檗胺 ( Ber)对高钾除极 ,Bay K8644,5-羟色胺 ( 5- HT)及咖啡因升高细胞内钙水平( [Ca2 + ]i)的影响。以 Fluo- 3/AM负载家兔培养的主动脉平滑肌细胞 ( VSMC) ,共聚焦显微术测定[Ca2 + ]i,结果以荧光强度 ( FI)表示 .结果 :( 1 )在细胞外钙为 1 .3mmol· L-1时 ,VSMC胞浆静息 FI明显高于核区 ,且不受 Ber的影响 . ( 2 ) Ber 1 0 -1 0 0 μmol·L-1预处理可抑制 KCl60 mmol·L-1或Bay K86441 0 0 μmol·L-1升高的 [Ca2 + ]i,抑制 5-HT 1μmol· L-1升高 [Ca2 + ]i 的持续相 ,但不影响[Ca2 + ]i 的一过性升高。维拉帕米 1 0 μmol· L-1具有相似作用 . ( 3)在无钙 Hanks液中 ,Ber预处理对咖啡因 1 0 0 mmol·L-1升高的 [Ca2 + ]i 无明显抑制作用。结果表明 ,Ber可阻断外钙内流 ,但不抑制内钙释放 ,这可能与 Ber阻断电压依赖性钙通道和受体依赖性钙通道的作用有关 .
The aim of present paper is to investigate the effects of berbamine (Ber) on cytosolic free calcium concentration ([Ca 2+ ] i) elevated by high K + depolarization, serotonin, and caffeine. Cultured vascular smooth muscle cell (VSMC) of rabbits was loaded with Fluo 3 AM, and [Ca 2+ ] i was measured in fluorescent intensity (FI) by confocal microscopy. The results showed that (1) in the presence of extracellular Ca 2+ 1.3 mmol·L -1 , the resting level of FI of [Ca 2+ ] i in cytoplasm was much higher than that in nucleus of VSMC and not affected by pretreatment with Ber. (2) Pretreatment of VSMC with Ber 10-30 μmol·L -1 , KCl 60 mmol·L -1 or Bay K8644 100 μmol·L -1 induced [Ca 2+ ] i elevation was inhibited (P<0.01). The sustained phase of [Ca 2+ ] i elevation by serotonin 1 μmol·L -1 was also reduced (P<0.01), while Ber did not work on the transient increase (P>0.05). The similar effects of Ber were also observed with verapamil 10 μmol·L -1 . (3) In D Hanks solution, [Ca 2+ ] i mobilization by caffeine 100 mmol·L -1 was not suppressed (P>0.05) by Ber pretreatment. The present data suggested that Ber could block the Ca 2+ influx probably due to both voltage dependent Ca 2+ channels and receptor operated Ca 2+ channels, but without the inhibitory effect on Ca 2+ release.
出处
《中国药理学与毒理学杂志》
CSCD
北大核心
2000年第2期96-100,共5页
Chinese Journal of Pharmacology and Toxicology
关键词
小檗胺
钙动员
共聚焦显微
主动脉
血管平滑肌
berbamine
calcium channels
cells, cultured
muscle, smooth, vascular
calcium,
