摘要
目的 探讨Snail在TGF-β1诱导人胃癌细胞MGC-803上皮细胞向间质细胞转化过程中的表达及其作用.方法 TGF-β1处理人胃癌细胞MGC-803不同时间（0h,3h,6h,24h,48h,72h）,倒置显微镜观察细胞形态的变化,Western blot方法检测Snail蛋白及E-cadherin,Vimentin蛋白的表达情况,Transwell实验检测细胞侵袭能力的变化.结果 （1）随着TGF-β1作用时间的延长,MGC-803细胞形态发生变化,伪足不同程度的增多;（2）在外源性TGF-β1作用下,胃癌细胞MGC-803均不同程度地表达Snail和E-cadherin,Snail从0h到6h表达量增强,到6h时达到最高.E-cadherin蛋白从0h到6h表达逐渐增高,6h时最高,后续变化不明显.Vimentin蛋白6h表达量最低,此后逐渐升高,72h时表达量最高;（3）Transwell小室实验显示6h时,细胞开始穿过ECM膜,随着时间的延长,穿过的细胞逐渐增多,72h最多.结论 在TGF-β1诱导人胃癌细胞MGC-803细胞EMT的过程中,Snail蛋白起到重要作用,启动Vimentin转录表达.
Objective To study Snail expression in epithelial- to- myofibroblast transition （EMT）of MGC - 803 cells induced by TGF -β1. Methods MGC - 803 cells were cultured in vitro. We observed cell morphological changes of MGC -803 cells at different times（0 h,3 h,6 h,24 h,48 h,72 h）after exposed to TGF -β1 from inverted microscope. Western blot method was employed to detect Snail, E - cadherin and Vimentin protein expressions. The transwell assay was used to observe the cell invasive ability. Result After being exposed to TGF -β1 for different times, the morphology of MGC - 803 cells was changed, and pseudopodia of different lev- els was increased. 2. Snail expression was increased from 0 h to 6 h（ the highest at 6 h）. E - cadherin expression was gradually increased from 0 h to 6 h （ the highest at 6 h ）. Vimentin expression was also gradually increased （the lowest at 6 h and the highest at 72 h）. Conclusion Snail proteins play an important role in the process of TGF-β1 induced EMT in MGC -803 cells ,which may induce vimentin protein transcriptional expression.
出处
《实用肿瘤学杂志》
CAS
2012年第2期141-145,共5页
Practical Oncology Journal
基金
黑龙江省自然基金（D200921）
