摘要
目的探讨microRNA-152(miR-152)在胃癌细胞中的表达情况以及对胃癌细胞增殖的影响及其可能的机制。方法采用实时荧光定量PCR(real-timePCR)方法检测胃癌细胞系MGC-803、SGC-7901、BGC-823和胃上皮细胞GES-1(作为对照)中miR-152的表达情况。采用脂质体法,转染miR-152模拟物(miR-152mimics)构建miR-152过表达体系,四甲基偶氮唑蓝(MTT)法检测miR-152对MGC-803胃癌细胞系增殖能力的影响,Westernblot检测转染miR-152mimics后E2F3蛋白的表达情况。结果 MGC-803、SGC-7901、BGC-823胃癌细胞系中miR-152的相对表达量分别为(0.05±0.01)、(0.19±0.03)、(0.48±0.09),表明在胃癌细胞中miR-152的表达明显下调(P<0.05)。MTT法检测结果显示,转染miR-152mimics后可明显抑制MGC-803胃癌细胞的增殖(P<0.05)。Westernblot结果表明,转染miR-152后,E2F3蛋白水平显著下降。结论 miR-152在胃癌细胞中明显低表达,并可能通过下调癌基因E2F3的表达,抑制肿瘤细胞的增殖,从而发挥抑癌基因的功能。
Objective To explore the expression of miR-152 in gastric cancer cell lines and the possible mechanisms on gastric cancer cell proliferation. Methods Real-time quantitative polymerase chain reaction （RT-qPCR） was used to quantify the expression level of miR-152 in human gastric cancer cell lines （MGC-803, SGC-7901, BGC-823） and one normal gastric epithelial cell line GES-1 （the control）. MGC-803 cells were transfected with miR-152 mimics to construct miR-152 over-expression vectors. After that, the proliferation of MGC-803cells was measured by MTT assay and the expression of E2F3 protein was identified by western blots. Results The expression of MiR-152 in gastric cancer cell lines MGC-803, SGC-7901, BGC-823 was （0.05±0.01）, （0.19±0.03）, （0.48±0.09）, indicating that the expression of MiR-152 was significantly down-regulated in gastric cancer cell lines, compared with GES-1 （P〈0.05）. After transfection of miR-152 mimics, the cell proliferation was inhibited （P〈0.05）, and the expression of E2F3 protein was down-regulated （P〈0.05）. Conclusion The expression of miR-152 is down-regulated significantly in gastric cancer cell lines. MiR-152 functions as a tumor suppressor and may inhibit cancer cell proliferation by down-regulation of E2F3.
出处
《海南医学》
CAS
2013年第7期943-946,共4页
Hainan Medical Journal
