摘要
以兜唇石斛的成熟蒴果作为外植体,比较不同的培养基、激素等因素对种子发芽及试管苗生根的影响。结果表明:以成熟蒴果为外植体能够诱导成苗,种子萌芽的最适培养基为:MS+NAA 0.5mg/L+6-BA 2.0mg/L+GA 1.0mg/L+香蕉汁150g/L+蔗糖20g/L+琼脂8g/L+活性炭2g/L,适宜的生根培养基为:1/4MS+NAA 2.0mg/L+6-BA 0.5mg/L+香蕉汁150g/L+蔗糖20g/L+琼脂8g/L+活性炭2g/L。
Taking mature capsule of Dendrobium aphyllum（Roxb.） C.E.C.Fisch as explant,the effect of different medium and phytohormones on seeds germination and root induction of seedlings of Dendrobiuma phyllum（Roxb.） C.E.C.Fisch.were studied.The results showed that seedlings could be induced from seed.The most appropriate medium for seedling inducing was MS＋NAA 0.5mg/L＋6-BA 2.0mg/L＋GA 1.0mg/L＋banana juice 150g/L＋sugar 20g/L＋ agar 8g/L＋activated carbon 2g/L.The most appropriate rooting medium was 1/4MS＋NAA 2.0mg/L＋6-BA 0.5 mg/L＋banana juice 150g/L＋sugar 20g/L＋agar 8g/L＋activated carbon 2g/L.
出处
《北方园艺》
CAS
北大核心
2012年第8期140-141,共2页
Northern Horticulture
基金
云南民族大学青年基金资助项目(11QN04)
云南省教育厅科学研究基金重点资助项目(2011Z005)
关键词
兜唇石斛
组织培养
种苗
培养基
Dendrobium aphyllum（Roxb.） C.E.C.Fisch
tissue culture
seedling
medium
