摘要
目的:探讨血管紧张素Ⅱ-2型受体(AT2R)A1675G基因多态性与家族性高血压的相关性。方法:选取高血压家系(高血压家系组)30个(120例受试者)和正常血压家系(正常血压家系组)29个(115例受试者)进行对照研究。采用聚合酶链式反应(PCR)技术分析AT2R A1675G基因多态性;采用酶联免疫吸附试验(ELISA)技术测定AT2R的表达。结果:高血压家系组AA型、AG型频率分布(53.3%、16.7%)比正常血压家系组(38.2%、12.2%)均升高,GG型频率分布(30.0%vs 49.6%)降低,高血压家系组比正常血压家系组等位基因A的频率分布(61.7%vs 44.3%)升高、G的频率分布(38.3%vs 55.7%)降低,差异均有统计学意义(P<0.05)。高血压家系组与正常血压家系组比较,AG型患者和GG型患者的AT2R表达浓度[(43.4±12.4)ng/ml vs(52.4±11.6)ng/ml,(53.2±15.3)ng/ml vs(65.9±16.9)ng/ml]均降低,差异有统计学意义(P<0.05)。结论:AT2R基因A1675G多态性与家族性高血压有明显相关性;G基因和AT2R在家族性高血压的发病中起着重要的保护作用。
Objective： To investigate the relationship between angiontensin II type 2 receptor（AT2R） gene A1675G polymorphism and familial hypertension. Methods： A pedigree based case-control study was conducted in two groups.The subjects with hypertension family history（HF＋） group,that including 30 pedigrees with 120 subjects,and he subjects without hypertension family history（HF-） group,that including 29 pedigrees with 115 subjects.AT2R gene A1675G polymorphism was detected by PCR,and AT2R protein expression was examined by ELISA. Results： HF＋ group had the increased AA,AG genotype frequencies than those in HF-group（53.3%,16.7%） vs.（38.2%,12.2%）,decreased GG frequencies（30.0% vs.49.6%）,increased allele frequencies of A（61.7% vs.44.3%）,decreased allele frequencies of G（38.3% vs.55.7%）,P0.05 respectively.The AT2R protein expression in the AG genotype and GG genotype of HF＋ group was lower than that in HF-group,（43.42±12.4） ng/ml vs.（52.35±11.6） ng/ml and（53.23±15.3）ng/ml vs.（65.9±16.9） ng/ml,P0.05 respectively. Conclusion： A1675G polymorphism in AT2R gene is obvious related to subjects with hypertension family history.G allele frequency and AT2R have the protective role in familial hypertension development.
出处
《中国循环杂志》
CSCD
北大核心
2012年第2期87-90,共4页
Chinese Circulation Journal
基金
山东省优秀中青年科学家奖励基金(批准文号:2007BS03003)
