摘要
目的:考察荜澄茄、干姜、辛夷挥发油对模型药磷酸川芎嗪的经皮渗透的影响。方法:用改良Franz扩散池法,以氮酮为对照,考察质量分数分别为1%,3%,5%,7%的荜澄茄、干姜、辛夷挥发油对模型药磷酸川芎嗪经小鼠皮肤的促渗效果,采用HPLC测定磷酸川芎嗪的含量。结果:5%氮酮,1%荜澄茄油,3%干姜油及5%辛夷油对磷酸川芎嗪的促透效果最好,对20 g·L-1磷酸川芎嗪12 h平均累计渗透量(Q12)分别为0.841 2,0.915 9,0.740 3,0.932 8 mg·cm-2.h-1;4 h时增渗倍数分别为1.357,1.279,1.054,1.156。经t检验,加入1%荜澄茄油的磷酸川芎嗪的Q12明显高于阴性对照组。结论:1%荜澄茄油、3%干姜油及5%辛夷油均能增加磷酸川芎嗪的经皮渗透量。
Objective：To investigate percutaneous penetration effect of different concentrations of volatile oil from Fructus Litseae（FL）,Rhizoma Zingiberis（RZ） and Flos Magnoliae（FM） on model drug-tetramethylpyrazine phosphate（TP）.Method： Modified Franz diffusion cell method was used,enhancing penetration effect through mouse skin of 1%,3%,5%,7% volatile oil extracted from FL,RZ and FM on LP were investigated with azone as comparison,the content of LP was determined by HPLC.Result： 5% azone,1% volatile oil from FL,3% volatile oil from RZ,5% volatile oil from FM had the best effect on enhancing permeation effect of LP through skin of mice,average cumulative permeation quantity on 20 g·L-1LP in 12 h（Q12）were 0.841 2,0.915 9,0.740 3,0.932 8 mg·cm-2·h-1,respectively;enhancing times in 4 h of each were 1.357,1.279,1.054,1.156,respectively.After t-test,Q12 of LP which added 1% volatile oil from FL was higher than the negative control group significantly.Conclusion： 1% volatile oil from FL,3% volatile oil from RZ and 5% volatile oil from FM could increase percutaneous permeation quantity of LP.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期1-4,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30960517)
云南省科技计划项目(2008CD152)
关键词
荜澄茄
干姜
辛夷
磷酸川芎嗪
经皮渗透
Fructus Litseae
Rhizoma Zingiberis
Flos Magnoliae
ligustrazine phosphate
percutaneous permeation
