摘要
目的:建立用高效液相色谱同时测定复方川芎胶囊中阿魏酸和川芎嗪含量的方法。方法:用Inertsil ODS2 C_(18)柱(150 mm×4.6 mm,5μm),流动相:甲醇-0.5%冰醋酸溶液(25:75),检测波长:280 nm,流速:1.0 ml·min^(-1)。结果:阿魏酸和川芎嗪的线性范围分别为4.4～44μg·ml^(-1)(r=0.999 9)和2.0～20.2μg·ml^(-1)(r=0.999 8),平均回收率分别为100.6% (RSD 2.1%)和100.9%(RSD 2.2%)(n=6)。结论:该法简单、快速、重复性好,可用于样品的含量测定及质量控制。
Objective： To establish an HPLC method for simultaneous determination the contents of ferulic acid and tetramethylpyrazine in compound chungxiong capsules. Method： The Inertsil ODS2 C18 column（ 150 mm× 4.6 mm,5 μm） was used, the methanol - 0.5 % glacial acetic acid solution （ 25： 75 ） as mobile phase, the flow rate was 1.0 ml·min^-1, the detection wavelength was 280 nm. Result： The linear ranges of ferulic acid and tetramethylpyrazine were at 4.4 - 44 μg·ml^- 1 （ r = 0. 999 9 ） and 2.0 - 20.2 μg· ml^-1 （r =0.999 8）, the average recoveries（n =6） were 100. 6% and 100.9% with RSD 2.1% and 2.2% respectively. Conclusion： The method is simple, rapid and repeatable, it can be used for quality control of compound chuangxiang capsules.
出处
《中国药师》
CAS
2008年第7期814-816,共3页
China Pharmacist
