摘要
采用线粒体DNA tRNAleu-COII片段的序列分析方法,对海南16个样点的715群中华蜜蜂(Apis cerana cerana)进行遗传多样性研究,明确了海南中华蜜蜂线粒体DNA tRNAleu-COII片段序列的遗传多样性及单倍型的分布特点.结果共发现48种单倍型,其中新发现43种单倍型.共检测到38个变异位点,包括12个单一多态位点、23个简约信息位点、1个插入位点和3个缺失位点.海南中华蜜蜂单倍型多样度为0.742±0.017,平均核苷酸差异数为1.248,核苷酸多样度为0.00354±0.00013,这表明海南中华蜜蜂线粒体DNA tRNAleu-COII片段具有丰富遗传多样性.海南中华蜜蜂普遍在117位点发生G→A的转换,发生比例为74.27%.
Genetic diversity of 715 colonies of Ap/s ceranct cerana from 16 sampling spots throughout Hainan Province in China were surveyed by mitochondrial DNA fragments of tRNA^leu-COII sequence. Totsl 48 haplotypes were found, 43 of which had not been previously published. There were 38 polymorphic sites, including 12 singleton variable sites, 23 parsimony informative sites, 1 insert site and 3 deletion sites. The average diversity of hapletypes was 0.742 ±0.017, the average nucleotide difference and the nucleotide diversity of the honeybee samples were 1.248 and 0.00354 ±0.00013, indicating the abundance of the haplotypes. There were 74.27% of honeybees in Hainan detected the transition in 117 site （G→A）. Honeybee introduced from the mainland and artificial rearing queen have led to the unstable stage of the genetic structure of Hainan honeybee in the influenced regions, that was why there was a significant difference genetic structures between sampling sites in the same region.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2012年第2期170-175,共6页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
现代农业蜂产业技术体系建设专项资金(CARS-45-KXJ11)
农业部热带农林有害生物入侵检测与控制重点开放实验室开放课题基金资助项目(MACKL1002)
关键词
海南
中华蜜蜂
线粒体DNA
遗传多样性
Hainan
Apis cerana cerana
mitochondrial DNA
genetic diversity
