摘要
Ⅰ型普鲁兰酶以内切的方式专一性作用于普鲁兰糖或者支链多糖的α-1,6糖苷键,生成产物麦芽三糖或线性多糖。在工业应用中,Ⅰ型普鲁兰酶与其他糖苷水解酶协同作用于不同糖类底物,可得到类型多样的终产物,因此在食品、化工、制药等行业中有很重要的作用。目前对Ⅰ型普鲁兰酶的研究不仅包括筛选高产酶的菌株,还需得到酶学性质优良的酶蛋白,以适合工业生产工艺。综述了细菌来源的Ⅰ型普鲁兰酶的研究概况,包括酶学性质、基因的分离与克隆、微生物生产,并讨论了该酶在工业生产中的应用及其研究前景。
TypeⅠpullulanase typically hydrolyzes the α-1,6 glucosidic linkages of pullulan or branched oligosaccharides in an endo-way to produce maltotriose or liner oligosaccharides,respectively.This enzyme plays a very significant role in the food,chemistry and medical industries,because they can degrade different saccharides with other glycoside hydrolases producing various products.At present,studies on this enzyme focus not only on screening novel strains,but also on obtaining type Ⅰ pullulanase with proper properties to suit industrial production.This review summarizes the research progress on type Ⅰ pullulanase from bacteria in some fields,such as catalytic properties,separation and cloning of gene,producing enzyme by microorganism.The industrial application of this enzyme and prospect of its research are also discussed.
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2012年第2期73-80,共8页
Journal of Agricultural Science and Technology
基金
中国农业科学院生物技术研究所基本科研业务费专项基金(1610392011008)资助
