摘要
本文用DEAE纤维素离子交换层析、ConA—Sepharose亲和层析、Sephadex G-100凝胶过滤等技术,分离纯化妊娠3～6周正常早孕妇女静脉血混合血清中早孕因子(Early Pregnancy Factor,EPF).以花结抑制实验检测各阶段层析物中EPF活性,以放射免疫法测定hCG含量.结果表明,经凝胶过滤后,提取物中hCG均为阴性(<3.1ng/ml),高EPF活性部分经SDS-聚丙烯酰胺凝胶电泳显示,分子量分别为24KD.和41KD_α。
An immunosuppressive early pregnancy factor (EPF) present in the serum of pregnent women, between 3 and 6 weeks of gestation, was isolated and purified using ion-exchange chromatography, affinity chromatography and gel filtration techniq(?) The serum samples and chromatographic fractions were measured for EPF activity using rossette inhibition test and hCG content by radioimmunoassay. The result showed that the column eluates (?) gel filtration exhibited no hCG (3. 1 ng/ ml). The fraction containing the bulk of EPF on SDS polyacrylamide gel electrophoresis revealed the presence of polypeptide bands of molecular weight at 24 kDa and 41 kDa respectively.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1990年第3期164-166,共3页
Chinese Journal of Immunology
关键词
早孕因子
孕血清
层析
Early pregnancy factor
Pregnancy serum
Chromatography
