摘要
用RFLP分析技术和分蘖株系法对由耐盐性品种Pokkali和盐敏感品种Peta配制的BC1 (Peta/Pokkali∥Peta)群体分别检测水稻苗期和成熟期耐盐性数量性状位点 (QTLs)。表型鉴定在含 (处理 )或不含 (对照 ) 6 0mol/m3NaCl的营养液中进行 ,苗期观测盐害级别、苗Na+ 含量和鲜重 /干重比值 3项指标 ,成熟期测定 1 0种农艺性状处理与对照的相对值。从水稻 1 2条染色体上筛选出 43个多态性标记 ,对上述指标分别作点分析 ,共检出 1 5个连锁标记。连锁标记的分布特点显示 ,在研究所涉及的基因组范围内存在 4个影响苗期耐盐性的QTL ,其增效等位基因均来自耐盐品种Pokkali;影响成熟期耐盐性的QTL分布于 7条染色体的 1或 2个连锁区间上 ,其有利基因来自双亲 ;RG6 78和RZ40 0B～RZ792附近的
Restriction fragment length polymorphism (RFLP) and tillered plant line assessment were employed to detect the quantitative trait loci (QTLs) for salt tolerance at different stages of rice development from a BC 1 [Peta/Pokkali(salt tolerant)∥Peta(salt sensitive)] population. Phenotypic evaluation was conducted in hydroponic culture with (treatment) or without (control) 60 mol/m 3 NaCl. Three indices (ranking of salt injury, Na + content in shoot and the ratio of shoot fresh weight/dry matter weight) at seedling stage and ten agronomic traits in control and salt treated plants at mature stage were measured. Single point analysis of 43 polymorphic markers screened from 12 chromosomes identified a total of 15 markers that were significantly associated with the indices mentioned above. The results from linkage analysis revealed: 1) There were 4 putative QTLs for salt tolerance at the seedling stage and all of the positive alleles came from Pokkali; 2) The QTLs for the salt tolerance at the mature stage distributed in one or two linkage regions on six chromosomes where the favorite alleles dispersed in both parents; 3) Two QTLs in the vicinity of RG678 and RZ400B RZ792 exhibited salt tolerance at both stages.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2000年第2期65-70,共6页
Chinese Journal of Rice Science
基金
广东省自然科学基金资助项目
美国洛克菲勒基金资助项目
关键词
水稻
数量性状位点
耐盐性
RFLP标记
rice
quantitative trait loci
restriction fragment length polymorphism
salt tolerance
