摘要
以云南马铃薯主要栽培品种为材料 ,使用进口PCR仪 ,对马铃薯RAPD分析中的DNA模板浓度、引物浓度、dNTP浓度以及PCR扩增反应循环次数进行了研究。结果表明适用于马铃薯RAPD反应的最佳条件为 :模板DNA1 5～ 5 0ng ,1 0 mer随机引物 2 5～ 5 0ng ,dNTP2 0 0 μM ,扩增反应周期 40～ 45个循环。每个循环包括 94℃变性 1min ,37℃退火 1min ,72℃延伸 2min。使用这一RAPD程序对云南的主要栽培品种进行品种鉴别 ,能有效区分出所有参试品种 ,检测出品种CFK6 9 1遗传背景特殊 ,较之于普通栽培品种亲缘关系较远。本实验建立了适宜马铃薯RAPD分析的PCR程序及条件 ,为RAPD应用于马铃薯的遗传研究打下了基础。
The objective of the present study was to analyse the potato RAPD protocol by using the main potato cultivars of Yunnan province.The results indicated that the optimum RAPD conditions for potatoes were:template concentration ranging 15 50ng;10 mer random primer 25 50ng;dNTP concentration 200μm;denaturing at 94℃ for 1 min,annealing at 37℃ for 1 min,extension at 72℃ for 2 min and the number of cycles from 40 to 45,All of the 11 cultivars were clearly identified by RAPD.The results indicated that the genetic background of cv. CFK69 1 was very special,its genetic distance was far from other cultivars.
出处
《西南农业学报》
CSCD
北大核心
2000年第1期59-64,共6页
Southwest China Journal of Agricultural Sciences
