摘要
利用ＰＣＲ－９０Ａ型ＤＮＡ扩增仪，对７个中国春小麦缺体核ＤＮＡ进行了扩增。结果显示，中国春小麦缺体核ＤＮＡ的ＲＡＰＤ产物可分为二种类型：（１）所有供试材料均能扩增出的片段，代表了核ＤＮＡ在进化上的保守性。有一个引物检测到这类片段。（２）全部供试材料间存在的差异片段，这类片段是用来基因定位的主要依据。另外。
RAPD analysis on seven nullisomic genomes of wheat “Chinese Spring”was conducted using four primers with 10mer oligonucleotides(OPT 13,OPT 14,OPT 6 and OPT 7).The RAPD products revealed that the amplfied DNA bands fell into two categories:those could be amplified in all genomes,represented the DNA stability in evolution;and those differed in the studied genomes,were the important bands for genome identification.Results also suggested that RAPD“fingerprints”assay of nuclear DNA could be used as a tool in the study of systematic evolution of wheat.
出处
《华北农学报》
CSCD
北大核心
1996年第3期31-34,共4页
Acta Agriculturae Boreali-Sinica
