摘要
目的:研究细胞色素P450表氧化酶对肿瘤坏死因子诱导的内皮细胞凋亡可能的机制。方法:原代培养的牛主动脉内皮细胞分为4组,分别转染细胞色素P450表氧化酶基因rAAV-GFP、rAAV-2J2、rAAV-2C11、rAAV-F87V病毒液,2周后用肿瘤坏死因子?α(TNF?α)(10ng/mL)和放线菌素D(ActD)(5ng/mL)诱导凋亡,GFP组不加TNF?α和ActD作为对照,另设空白对照组,用Western blot法检测Bcl-2和Bax的表达,并用酶标法检测Caspase-3的活性。结果:Western blot结果表明转染CYP P450表氧化酶的各组后Bcl-2表达升高,Bax表达下降,Caspase-3的活性下降,较空白对照组及GFP对照组差异有统计学意义(P均<0.01)。结论:细胞色素P450抗内皮细胞凋亡的机制可能与上调Bcl-2表达,下调Bax表达及抑制Caspase-3活性有关。
Objective： To study the protective effect of arachidonic acid cytochrome P450 epoxygenases（CYP450） on the apoptosis of endothelial cells induced by tumor necrosis factor alpha.Methods：Arachidonic acids CYP450 genes were transfected by CYP2J2,CYPF87V,CYP2C11 using recombinant adeno-associated viral vector into cultured bovine aortic endothelial cells for 2 weeks.The level of Bcl-2 and Bax were detected by western blot.The activity of caspase-3 was detected by colorimetric assay.Results：Expoxygenases enhanced the expression of Bcl-2,reduced the expression of Bax,and inhibitd the activity of caspase-3.Conclusions：Arachidonic acid CYP450 reduces the apoptosis of endothelial cells and Bcl-2/Bax and caspase-3 may take part in the control of apoptotic process.
出处
《海南医学院学报》
CAS
2012年第4期441-444,共4页
Journal of Hainan Medical University
基金
国家自然科学基金资助课题(30270561)~~
