摘要
通过单因素和均匀设计对巨大口蘑液体菌种发酵工艺条件进行了优化,研究结果表明,巨大口蘑液体发酵最佳条件为:装液量179mL、摇床转速165r/min、发酵温度28℃、初始pH6、接种量为4片直径1.2cm的菌丝块,干菌丝量达到0.8626g。通过实验研究,得到了巨大口蘑液体发酵的最佳条件,为今后巨大口蘑液体菌种的生产提供理论依据。
The optimization of liquid shake-flask fermentation conditions of Tricholoma giganteum was studied by single factor experiment and uniform design method. The results showed that the optimal liquid shake-flask fermentation conditions Tricholoma giganteum were as follows.volume was 179mL medium in 250mL flask, rotate speed was 165r/min,cultivation temperature was 28℃, pH was 6 and the inoculum size was 4 pieces of 1.2cm sclertium in diameter. The weight of dry mycelia achieved to 0.8626g. Through the experiment research, liquid optimal fermentation conditions of Tricholoma giganteum was obtained,theoretical basis would be provided for the future Tricholoma giganteum liquid strain production.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第8期228-231,共4页
Science and Technology of Food Industry
基金
广东省科技计划项目(2011B020303007)
广东省科技特派员专项项目(2010A02050700-18)
关键词
巨大口蘑
发酵
均匀设计
Tricholoma oiaanteum
fermentation
uniform desian
