摘要
用高效液相色谱法分离并建立了复方乙酰水杨酸片中阿司匹林、非那西丁和咖啡因含量的测定方法。色谱分析条件分别为:色谱柱为Kromasil C18(4.6mm×250mm,5μm),柱温为35℃;流动相为甲醇-1%冰醋酸(45∶55),流速为1.0mL/min。检测波长为276nm。进样量为20μL,外标法定量。在该色谱条件下,阿司匹林、非那西丁及咖啡因分离度均大于1.5,且浓度与峰面积呈良好的线性关系,线性范围分别为4～960μg/mL(R2=0.999 9)、4～200μg/mL(R2=0.999 9)及4～200μg/mL(R2=0.999 9),平均加标回收率分别为99.2%(RSD=1.2%)、100.4%(RSD=1.4%)及101.1%(RSD=0.9%)。所建立的方法简单、灵敏、准确,可用于复方乙酰水杨酸片的质量控制。
The present research is to establish a HPLC method for the determination of aspirin,phenacetin and caffeine in compound aspirin tablets.In this method,a Kromasil C18 column（4.6 mm×250 mm,5 μm） was used and the mobile phase was methanol-1% acetic acid solution（45∶55） with the flow rate of 1.0 mL·min-1.The UV detection wavelength was 276 nm and the column temperature was 35 ℃.The injection volume was 20 μL and external standard method was adopted.The calibration curves were linear in the range of 4~960 μg/mL（R2=0.999 9）for aspirin,4~200 μg/mL（R2=0.999 9） for phenacetin,and 4~200 μg/mL（R2=0.999 9）for caffeine.The average recoveries are 99.2%,100.4%,101.1% for the three components respectively with RSD of 1.2%,1.4%,0.9%.The method is simple,sensitive,accurate,and can be applied applicable to the analysis of compound aspirin tablets.
出处
《北京石油化工学院学报》
2012年第1期41-44,共4页
Journal of Beijing Institute of Petrochemical Technology
基金
北京市大学生科研训练(URT)项目
项目号:2011J00053
