摘要
目的:建立一种快速、准确、灵敏的测定血清中维生素K(3VK3)的电化学方法。方法:镀汞膜修饰玻碳电极为工作电极,Ag/AgCl电极为参比电极,铂片电极为辅助电极,组成三电极体系,在pH=9.4、浓度为1.0 mol.L-1氨水-氯化铵缓冲液的底液中,用差分脉冲溶出伏安法对血清中VK3进行测定。结果:峰电流(ip)与VK3的浓度(C)在0.23～1.6 ng.mL-1范围内呈现良好的线性关系,检出限为0.23 ng.mL-1,并具有很好的平行性,对于0.92 ng.mL-1的VK3,6次平行测定结果的标准偏差RSD为1.64%。应用此方法对血清中VK3含量进行了测定,方法的平均回收率为99.91%,RSD为1.03%。结论:该方法与高效液相色谱法相比简单、快速、灵敏度高,可作为血清中VK3的高敏检测方法。
Objective： To establish a new accurate,sensitive and simple electrochemical method for the determination of vitamin K 3 in serum.Methods： There was a three electrodes system with glassy carbon as working electrode,Ag/AgCl as reference electrode,Pt as auxiliary electrode.With the three electrodes system,the content of vitamin K 3 standard solution was determined by the method of differentinal pluse stripping voltammertry in NH 3.H 2 O/NH 4 Cl buffer which pH was 9.4 and concentration was 1.0 mol.L-1.Results： A good linear relationship between the stripping peak current and vitamin K 3 concentration in range of 0.23～1.6 ng.mL-1 was held（r=0.999 4）,with a detection limit of 0.23 ng.mL-1.RSD was1.64% for the determination of vitamin K 3 at level of 0.92 ng.mL-1（6 次）.The recovery rate was 99.91%.Conclusion： Compared with the method of high pressure liquid chromatography（HPLC）,it is simple,rapid,sensitive,and it can be a high sensitive method for the determination of vitamin K 3 in serum.
出处
《天津医科大学学报》
2012年第1期125-128,共4页
Journal of Tianjin Medical University
基金
科技型中小企业技术创新资助项目(10C26211203586)
