摘要
单扫描示波极谱法和新极谱法(卷积伏安法)都可检测维生素K_3,且不经分离就可以达1×10^(-8)mol·L^(-1)(3.51ng/ml),适用于临床分析。用0.1mol·L^(-1) NaClO_4+0.01mol·L^(-1) HClO_4为底液时,浓度1×10^(-8)～5×10^(-5)mol·L^(-1)(3.51ng/ml～17.5μg/ml),可作定量分析。25℃时,前法的Ep=-0.78V(SCE),检测限为5×10^(-9)mol·L^(-1)(1.75ng/ml);后法则为-0.76V(Ag/AgCl)及4×10^(-9)mol·L^(-1)(1.40ng/ml)。将本法应用于检定针剂和动物血浆中的V_(K3),得到满意的结果。还通过多种方法和试验证明其电极过程为扩散控制的不可逆的连续单电子的还原过程(n=2)。
Vitamin K_3can be detected or determined either by the single sweep oscillopolarographic method or by the neopolarographic method (convolution voltammetry). The extraction procedure was not necessary in clinical trail. Using a base solution of 0.1mol/L NaClO_4 -0.01mol/L HClO_4, there is a linear relation between the peak height (i_p' or e_p') and concentration within the range 1×10^(-8)—5×10^(-5) mol/L (3.51ng/ml—17.5μg/ml). As a result, this linearity can be used for quantitative analysis. At 25℃ the respective peak potentials and the detection limits are -0.78V (SCE), 5×10^(-9) mol/L (1.75ng/ml), and -0.76V (Ag/ AgCl), 4×10^(-9) mol/L (1.40ng/ml) for two methods. The samples of injection vitamin K_3 and plasma vitamin K_3 were determined directly by standard addition method and calibration curve method. All experiments were finished in an hour. Besides, the electrode process is reversible in two steps of single electron reduction controlled by diffusion.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1989年第4期312-316,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
