摘要
试验研究了用丙酮溶液提取饲料中单宁,溶液经过滤后,取滤液加钨酸钠-磷钼酸混合溶液,显色后,在760 nm波长处测定吸光度值,标准曲线法测定饲料中单宁含量。单宁在0.50～6.00 mg/l浓度范围内与吸光度值呈良好的线性关系,线性回归方程为A=0.056 48×C+0.001 08,相关系数为0.999 7,方法的检出限为17.5 mg/kg。该方法操作简便、快速、结果准确,应用于饲料中单宁的测定,结果满意。
The optimum condition of determination of tannin in feeds using spectrophotometric method with sodium tungstate-molybdphosphoric acid was studied.The linear range of the method for determination of tannin was from 0.50 to 6.00 mg/l and the limit of detection was 17.5 mg/kg.The method was simple,rapid and reliable for the determination of tannin in feeds.
出处
《饲料工业》
北大核心
2012年第7期51-55,共5页
Feed Industry
关键词
比色分光光度法
单宁测定
饲料
spectrophotometry method
determination of tannin
feeds
