摘要
从新疆阿勒泰地区哈萨克族传统发酵乳制品中分离得到11株乳酸菌,采用生理生化特性和16S rDNA序列同源性分析,对其进行鉴定。通过纸片法筛选,大多数乳酸菌对大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)、铜绿假单胞菌(Pseudomonas aerugonosa)、枯草芽孢杆菌(Bacillus subtilis)和李斯特氏菌(Listeria monocytogenes)这5株指示菌有抑菌效果,其中1株干酪乳杆菌(TNG2)和4株瑞士乳杆菌(NS2-a,TNS1-1,NS1-1和NS2-1)对5株指示菌均有抑菌效果。
Based on characteristics of physiological and biochemical and 16S rDNA gene sequences eleven lactic acid bacteria isolated from Kazakh traditional fermented dairy products of Altay was identified. Screened by disk diffusion method, most of the lactic acid bacteria showed inhibiting the growth of five indicator strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aerugonosa, Bacillus subtilis, Listeria monocytogenes. Five strains of lactic acid bacteria identified as Lactobacillus casei ( TNG2 ) and Lactobacillu helveticus ( NS2-a, TNS 1-1, NS 1-1 and NS2-1 ) showed inhibition to all indicator strains.
出处
《食品工业》
北大核心
2012年第4期109-111,共3页
The Food Industry
基金
国家自然科学基金(项目编号:30960001)
新疆生产建设兵团青年科技创新资金专项基金项目(项目编号:2011CB001)
石河子大学研究生创新基金项目(项目编号:YJCX2010-Y14)
关键词
传统乳制品
乳酸菌
生理生化
16S
RDNA
抑菌
traditional dairy products
Lactic acid bacteria
physiological and biochemical
16S rDNA
antibacterial