摘要
以橡胶草叶片为外植体,研究了消毒时间、外源激素等因素对外植体成活率、不定芽的诱导、伸长和生根的影响。结果表明:用0.1%的升汞处理7min为最佳消毒方法,最佳的再生培养基为MS+1.5mg/L 6-BA+0.1mg/L NAA,平均诱导率为97.3%,芽伸长培养基为MS+2.0mg/L 6-BA+0.02mg/L NAA+0.4mg/L GA3,生根培养基为1/2MS+0.2mg/L NAA,生根率为100%,所获得的组培苗生长健壮且移栽成活率高,最终摸索出了橡胶草最佳的再生体系。
The effects of disinfection time and exogenous hormones on the survival rate,induction of in vitro shoot,elongation and root formation were studied by taking young leaf of Taraxacum kok-saghyz Rodin as explants.The results showed that the optimum disinfection method was treated with 0.1% HgCl2 for 7 min;the optimum regeneration medium was MS＋1.5 mg/L 6-BA＋0.1 mg/L NAA and the average induction rate was 97.3%;the optimum shoot regeneration medium was MS＋2.0 mg/L 6-BA＋0.02 mg/L NAA＋0.4 mg/L GA3;the best rooting medium was 1/2MS＋0.2 mg/L NAA,the rooting rate was 100%,the tissue cultured plants obtained grew well and had high survival rate after transplanting,the optimum regeneration system of Taraxacum kok-saghyz Rodin was found finally.
出处
《北方园艺》
CAS
北大核心
2012年第7期115-119,共5页
Northern Horticulture
关键词
橡胶草
再生体系
生根
Taraxacum kok-saghyz Rodin
regeneration system
rooting
