摘要
探讨了朱砂根继代培养中抗氧化剂(维生素C、L-半胱氨酸、硫代硫酸钠)和吸附剂(活性炭、聚乙烯吡咯烷酮)对外植体褐化的影响。结果表明:培养基中添加活性炭3 g.L-1,继代周期控制在30 d之内,平均褐化率可控制在18.89%,效果最理想;培养基中添加维生素C 0.2 g.L-1也能有效控制褐化,但整体效果不如活性炭。
The paper researched the prevention of browning in tissue culture of Ardisia crenata Sims with the effect of application of anti-browning agents,including 3 antioxidants(vitamin C,L-cysteine,Na2S2O3)and 2 absorbents(activated carbon,Polyvinyl Pyrrolidone).The results showed that,when 3 g·L-1 of activated carbon was added to medium and the subculture cycle was controlled in 30 days,the browning rate could effectively reduced to 18.89%,which was the best treatment in this study.The browning was also controlled by adding 0.2 g·L-1 of vitamin C to medium,however,the effect of browning control of vitamin C was not as good as the activated carbon's.
出处
《福建林业科技》
北大核心
2013年第1期99-102,共4页
Journal of Fujian Forestry Science and Technology
基金
龙岩市科技计划项目(项目编号:2009LY19)
关键词
朱砂根
继代培养
褐化控制
Ardisia crenata Sims
subculture
browning control
