摘要
目的建立回肠-盲肠侧侧吻合术导致的实验性末端回肠炎动物模型以及双歧杆菌干预模型,观察Toll样受体(TLR)2和TLR4在双歧杆菌干预下的表达,研究双歧杆菌对实验性末端回肠炎的保护作用。方法清洁级SD雄性大鼠100只,随机分为五组:正常对照组(A)、模型对照组(B)、双歧杆菌低剂量干预组(C)、中剂量干预组(D)及高剂量干预组(E),每组20只。观察这五组大鼠末端回肠的炎症情况,行镜下肠组织病理学评分(HS),使用免疫组化法检测各组末端回肠黏膜中TLR2、TLR4的表达。结果 (1)肉眼观察,模型对照组与双歧杆菌干预组及空白对照组相比,炎症评分有统计学差异(P<0.05)。(2)镜下观察,模型对照组与双歧杆菌干预组及空白对照组相比,炎症评分差异有统计学意义(P<0.05)。(3)造模对照组肠上皮细胞TLR2和TLR4的平均吸光度值较其余四组高(P<0.05);在双歧杆菌干预下,干预组TLR2和TLR4的平均吸光度值低于B组(P<0.05)。结论使用双歧杆菌干预可降低实验性末端回肠炎肠组织TLR2、TLR4的表达,双歧杆菌对实验性末端回肠炎有保护作用。
Objective To establish the ileum-caucus side-to-side intestinal anatomists induced SD mouse experimental terminal ileitis（ETI）model and the bifid bacteria intersection model,to study the expression of TLR2 and TLR4 in ETI with or without bifid bacteria and study bifid bacteria′s effects in ETI.Methods One hundred healthy male SD rats were randomly assigned into five groups,namely the normal central group（A）,the ETI model group（B）,the low bifid bacteria given group（C）,the middle bifid bacteria given group（D）and the high bifid bacteria given group（E）.Firstly,the experimental groups were observed the tissue′s general macroscopic view,then the tissues were fixed 10% neutral formalin,taken HE and imunohistochemistry stain,embedded in paraffin for blinded histological analysis,to observe the positive expression of TLR2 and TLR4.Results Compared B with other groups the HS score was significant different at the same time（P〈0.05）.Compared B with other groups TLR2 and TLR4 positive expression were increased（P〈0.05）,but the positive expressions of group C-E TLR2 and TLR4 were lower than group B（P〈0.05）.Conclusions Using bifid bacteria can significantly reduce the expression of TLR2 and TLR4 in ETI intestinal tissue,bifid bacteria has a protective effect in rat of ETI.
出处
《中华临床医师杂志（电子版）》
CAS
2012年第5期78-81,共4页
Chinese Journal of Clinicians(Electronic Edition)
