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IL-6对肥大细胞Toll样受体4表达及细胞因子释放的调节 被引量：2

Modulation effect of IL-6on Toll-like receptor 4expression and cytokine release in mast cells
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摘要 目的探讨IL-6对肥大细胞Toll样受体4(TLR4)表达的调节作用。方法不同浓度IL-6及其阻断抗体作用P815细胞2、6、16h后,采用流式细胞术和实时定量PCR检测TLR4表达变化,ELISA法检测细胞因子释放情况。结果 IL-6呈剂量和时间依赖性地上调P815细胞TLR4的表达及细胞因子IL-4的释放,而IL-6阻断抗体能阻断这个增加过程。结论 IL-6促进TLR4的表达及细胞因子的释放,为进一步研究肥大细胞TLR4参与变态反应提供了新思路。 Objective To investigate the regulatory effect of IL-6 on toll-like receptor 4（TLR4） expression and cytokine release in mast cells. Methods P815 cells were stimulated by different concentrations of IL-6 and its blocking antibody for 2,6 and 16 11. TLR4 expression was detected by flow cytometry and RQ-PCR, and cytokine release in mast cells was determined by ELISA. Results IL-6 upregulated TLR4 expression and IL-4 release in the time- and dose-dependent manners in mast cells,which was reversed by IL-6 blocking antibody. Conclusion IL-6 can promote TLR4 expression and cytokine release,which may provide a new clue for further investigation of allergic reaction.
作者 朱晓敏 杨海伟 魏继福 何韶衡
机构地区 南京医科大学第一附属医院临床实验研究室
出处 《江苏医药》 CAS 北大核心 2013年第22期2665-2667,共3页 Jiangsu Medical Journal
基金 国家自然科学基金(30972822 81001329 81172736 81030054 81273274) 江苏省"科技兴卫工程"医学重点学科开放课题(XK12200902) 江苏高校优势学科建设工程资助项目
关键词 TOLL样受体4 白细胞介素 肥大细胞 Toll-like receptor 4 Interleukin Mast cells
分类号 R392 [医药卫生—免疫学]
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参考文献9

  • 1杨海伟,魏继福,何韶衡.肥大细胞Toll样受体的研究进展[J].细胞与分子免疫学杂志,2008,24(9):933-934.
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二级参考文献18

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江苏医药
2013年 第22期

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