摘要
突变型黑曲霉（Ａｓｐｅｒｇｉｌｌｒｓ ｎｉｇｅｒ）Ｍ００１分别经紫外线（ＵＶ）、亚消基胍（ＮＴＧ）、ＴＤＰ辐射仪及空间微重力辐射（ＳＣ）等因素的多级循环处理，得到１株形态发生改变的变异菌株Ｘ－１５。其固体培养纤维素酶的滤纸分解酶活力（ＦＰＡ）为１９Ｕ／g，羟甲基纤维素酶（ＣＭＣａｓｅ）活力为４５３Ｕ／g，β－葡萄糖苷酶（β－ｇｌｕａｓｅ）活力为１５４Ｕ／g，与出发菌株Ｍ００１相比，分别为其２．８倍、
A mutant strain X 15 with high activity of cellulase was obtained from parent strain of A.niger M001 by cyclic treatment of UV, NTG, TDP irradiator and space conditions. The optimum conditions of solid fermentation for cellulase production are grass meal 70 g, wheat bran 30g, tap water 150 200 mL, (NH 4) 2SO 4 2 g, pH6.0 at 30℃ for 96 hours. Under those conditions, its enzyme activities toward filter paper, CMC and salicin degradation reached 19, 453 and 154 U/g and increased by 2.8, 2.2 and 4.1 times, respectively, as compared to parent strain. [WT5HZ]
出处
《中国兽医学报》
CAS
CSCD
北大核心
2000年第1期97-99,共3页
Chinese Journal of Veterinary Science
基金
全军医药卫生科研基金
