摘要
研究了康氏木霉B－7和黑曲霉X－152株纤维素酶高产菌株的原生质体制备与再生。结果表明，采用纤维素酶、蜗牛酶、溶菌酶的混合酶液，可成功地制备2株真菌的原生质体。其中，B－7以这3种酶的配比6：5：2为最佳，X－15以8：4：2为最佳。原生质体形成的缓冲液系统均以0．2mol/L，pH6．0磷酸盐缓冲液为宜，渗透压稳定剂则分别以0．6mol/LNaCl和0．6mol/L蔗糖为宜。以菌龄18h（B-7）和16h（X－15）2株真菌的菌丝体，在37℃下酶解90min可获得最适量的原生质体，产量分别达9×106个／ml和1．9×107个／ml，且其再生率也较高，均达95％左右。
The preparation and regeneration of protoplasts from two high - yield cellulase - produc-ing fungal strains, Trichoderma koningii B - 7 and Aspergillus nipe X - 15 were obtainedsuccessfully by using a mixed enzyme soution of cellulase, snail enzpe, and lysozyme. Theoptimum proportion of the enzymes were 6: 5: 2 (B - 7) and 8: 4: 2 (X -15) respectively.Phosphate buffer (0. 2mol/L at pH 6. 0) was suitable buffer for protopast formation. Silu-tion of 0. 6mol/L of NaCl for B - 7 and 0. 6mol/L of sucme for X - 15 were the suitable os-motic stabilizer respectively. The mycelia of 18h old for B - 7 and 16h old for X - 15 could beenzyme- digested in 90min at 37℃, and just to the right amount of protoplasts were ob-tained. The yields were up to 9X106/ml (B - 7), and 1. 9X107/ml (X - 15) respectively.And the regeneration rate of the protolasts was also high, it was around 95%.
出处
《微生物学杂志》
CAS
CSCD
1999年第1期19-23,共5页
Journal of Microbiology
基金
吉林省科委资助
关键词
康氏木霉
黑曲霉
原生质体形成和再生
Trichoderma koningii
Aspergillus niger
formation and regeneration of protoplasts
