摘要
目的:建立HPLC测定葛根饮片中3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷元-8-C-芹菜糖-(1→6)葡萄糖苷和大豆苷5个成分含量的方法。方法:采用Zorbax C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.02%磷酸(25∶75)为流动相,检测波长250 nm。结果:方法学考察合格,并测定了12批葛根饮片中5个成分的含量。结论:该方法简便、快速、准确度高,可作为葛根饮片的质量标准。
Objective：To establish a HPLC method for determination of 3′-hydroxy puerarin,puerarin,3′-methoxy puerarin,daidzein-8-c-apiosyl-（1→6） glucoside and daidzin in radix Pueraria lobata.Method： A Zorbax C18（4.6 mm×250 mm,5 μm） was used;the mobile phase was methanol-0.02% phosphoric acid（25∶75）.The detection wavelength was at 250 nm;the flow rate was 1.0 mL·min-1 and the column temperature was maintained at 40 ℃.Result： The accuracy,precision,sensitivity,specificity and linearity of this method met the requirements.The method was applied to 12 batches of radix Pueraria lobata.Conclusion： The method is rapid,simple and accurate,which can be used in the quality control in radix Pueraria lobata.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第7期108-111,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中药质量控制技术国家工程实验室课题(2010DZHT001)
