摘要
目的 研究成纤维细胞激活蛋白（FAP）在乙肝相关性肝癌中的表达,探讨其临床意义.方法 采用反转录聚合酶链反应（RT-PCR）检测33例乙肝相关性肝癌患者手术切除肝癌组织、癌旁组织及远隔相对正常肝组织及13例正常肝组织FAP表达情况,同时采用荧光定量PCR方法检测其mRNA表达差异.结果 FAP mRNA在肝癌组织、癌旁组织以及远隔相对正常肝组织中均有表达,相对于对照组正常肝组织的差异倍数值分别为5.14 ±6.69、1.58±0.96、1.63 ±0.94,三者比较差异有统计学意义（F=4.401,P＜0.05）；FAP mRNA的差异倍数值在Ⅰ期、Ⅱ期、Ⅲ期肝癌中分别为2.89±3.35、4.15±4.69、10.09 ±9.51,在高、中、低分化肝癌中分别为1.62±1.74、3.84±3.79、11.26±13.34,差异均有统计学意义（P＜0.05）.结论 FAP可能在乙肝相关性肝癌的发生、发展中发挥重要作用,可作为乙肝相关性肝癌分化程度及预后判断的指标.
Objective To analyze the gene expression level of fibroblast activation protein in HBV related hepatocellular carcinoma patients and discuss its clinical significance.Methods FAP gene expression in 33 hepatocellular carcinoma patients cancer tissues,peficancerous tissues,distant relative normal liver tissues and 13 normal liver tissues were examined by reverse transcription PCR;and real-time fluorescent quantitative PCR （qRT-PCR） was used to quantify their expression.Results FAP were expressed in all the tissues,the relative expression values in cancer tissues,peficancerous tissues and distant relative normal liver tissues were 5.14 ± 6.69,1.58 ± 0.96,1.63 ± 0.94,respectively,the differences were statistically significant （ F =4.401,P 〈 0.05 ） ; and in TN M stage Ⅰ,Ⅱ,Ⅲ,they were 2.89 ± 3.35,4.15 ±4.69,10.09 ± 9.51 respectively; in well - differentiated,differentiated and poorly differentiated hepatocellular carcinoma were 1.62 ± 1.74,3.84 ±3.79,11.26 ± 13.34respectively.The differences were all statistically significant（ P 〈 0.05 ）.Conclusion FAP may play an important role in the occurrence and development of HBV related hepatocellular carcinoma.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2011年第6期463-465,共3页
Chinese Journal of Experimental and Clinical Virology
基金
基金项目：人事部留学回国人员择优资助项目：HBV慢性感染及HCC临床发病机理和治疗研究
教育部新世纪优秀人才支持计划资助项目（NCET-04-0797）
关键词
癌
肝细胞
肝炎病毒
乙型
成纤维细胞生长因子
Carcinoma,hepatocellular
Hepatitis B virus
Fibroblast growth factors
Hepatocytes
