摘要
对87份青稞和13份野生大麦进行SDS-PAGE分析,结果发现,供试材料的HMW-GS条带单一,大多只有1条带,个别材料有2条带,而且条带的位置也变化不大,仅有3种条带位置,且基本介于小麦HMW-GS的7亚基至12亚基位置之间。因此,根据条带迁移位置的不同对参试材料的3种条带命名为A,B,C,分别对应着A,B,C亚基。87份青稞材料共出现了A,B,C,AC等4种带型,分别对应着56,1,27,3号青稞材料,各带型分别占64.4%,1.1%,31.0%,3.4%;其中A,B,C为单带带型,AC为双带带型;出现AC带型的原因是材料中同时存在A带型和C带型的种子。青稞和野生大麦高分子量麦谷蛋白亚基类型较单一,变异不丰富,多态性较低。本试验对青稞高分子量麦谷蛋白亚基多态性的研究可为其面粉加工利用和品质改良提供科学依据。
87 hulless barley varieties（strains） and 13 wild barley accessions were analyzed by SDS-PAGE.The result showed that the HMW-GS bands were simple and lack of variance,with most materials had just one band and few materials had two bands;only 3 band positions in total occurred almost between 7 subunit and 12 subunit of Chinese Spring,a reference wheat cultivar.Thus the temporary names were given according to their respective migration distance in gel by comparing with the bands of the wheat cultivar Chinese Spring（CS）： the band slightly lower than the 7 subunit of CS was named as A band;the band slightly lower than A band and much higher than 8 subunit of CS was named as B band;the band between 8 and 12 subunit of CS was named as C band.A,B and C band corresponded to A,B and C subunit,respectively.Four band patterns,A,B,C and AC were detected in 87 hull-less barley materials with each pattern containing 56,1,27 and 3 accessions and taking the ratio of 64.4%,1.1%,31.0% and 3.4%,correspondingly.A,B and C pattern were single band patterns,and AC pattern was double band pattern;the AC pattern occurred when A pattern seeds and C pattern seeds existed in one material.These data indicates that the HMW-GS in hulless barley and wild barley may have single composing,lacking variation and lower polymorphism.The polymorphism study of high molecular weight glutenin subunits（HMW-GS） in hulless barley can provide the basis for the flour milling and quality improving.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第2期145-149,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
国家现代农业产业技术体系建设项目(CARS-05)
引进先进农业科学技术计划项目(“948”计划)(2010-Z29)
