摘要
目的探讨动物宿主棘球蚴感染的检测方法,为绦虫感染的治疗与预防提供参考。方法选择泡状棘球蚴病羊血清和囊型棘球蚴病羊血清各100份,建立AgB原核表达体系,采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)分析及酶联免疫吸附测定(ELISA)进行血清学诊断评价。结果联合表达抗原AgBs对囊型棘球蚴病羊血清的敏感性和特异性均为90.0%,明显高于AgB1对囊型棘球蚴病羊血清的敏感性和特异性(分别为70.0%、56.0%)(P<0.05)。结论联合表达抗原AgBs对囊型棘球蚴病的诊断价值优于AgB1。
Objective To explore the detection methods of echinococcus infection in animal host and provide a reference for prevention and treatment of tapeworm infection.Methods Serum samples of goats with cystic echinococcosis and with alveolar echinococcosis（each of 100） were collected.AgB prokaryotic expression system was established.Sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE） was employed to analyze AgB expression and enzyme-linked immunosorbent assay（ELISA） was adopted to evaluate the serological diagnosis.Results Sensitivity and Specificity of co-expression antigen AgBs to sera of goats with cystic echinococcosis were both 90.0%,which were higher than those of AgB1（70.0% and 56.0%,respectively）（P0.05）.Conclusion The diagnostic value of co-expression antigen AgBs in cystic echinococcosis is prior to that of antigen AgB1.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第9期867-868,871,共3页
Chongqing medicine
关键词
棘球蚴病
敏感性与特异性
诊断技术和方法
echinococcosis
sensitivity and specificity
diagnostic techniques and procedures
