摘要
本文用ABC-ELISA法比较研究了HHF和GAH抗原对71例泡球蚴病患者、107例非泡球蚴病患者和86例健康人的诊断价值。结果表明:(1)在以S/N≥2.2为阳性标准时,泡球蚴患者用HHF和GAH抗原的敏感性相同,均为98.59％,假阳性率前者为3.63％,后者为2.07％,经统计学处理两者差别不显著(t=0.92);(2)泡球蚴患者的S/N值在6.0以上者,HHF抗原占67.61％,非常显著地高于GAH的36.62％(t=3.89),棘球蚴患者的HHF抗原占59.75％,也非常显著地高于GAH抗原的6.09％(t=8.91);(3)泡球蚴患者的ABC-ELISA抗体滴度在1∶6400以上者,HHF和GAH抗原各占67.61％和74.61％,两者无显著差别,说明同种和异种抗原均可用于泡球蚴病的诊断;(4)泡球蚴病的ABC—ELISA抗体几何平均滴度用在GAH抗原时为1∶8577.39,为用HHF抗原(1∶7555.04)的1.14倍,而棘球蚴病的抗体几何平均滴度在HHF抗原为1∶2891.39,为GAH抗原(1∶1252.15)的2.31倍,说明在诊断上宜用同种抗原;(5)泡球蚴患者的抗体几何平均滴度比棘球蚴患者高,在用HHF抗原时前者为后者的2.61倍,GAH抗原前者为后者的6.85倍。
A comparison was made of the diagnostic value of antigens from human hydatid fluids (HHF) and gerbil alveolar hydatid (GAH) in sera from 71 patients with multilocular echimococcosis, 107 with other diseases and 86 healthy adults by ABC-ELISA . The results indicated that (1) when the sera were 1 :200 diluted and an S/N 2.2 was taken as positive, the sensitivity of the ABC-ELISA with antigens from HHF and GAH in patients with alveolar disease were 98.59% .The difference in the false positive reactions of the tests between HHF (3.63 % ) and GAH (2.07 %) antigens was not significant (t = 0.92) . (2) In 67.61% of alveolar hydatidosis with HHF antigen, the value of S/N was above 6.0, it was significantly higher than that of GAH antigens (36.62%) (t = 3.89) , and in patients with unil-ocular hydatids, the HHF and GAH antigens was 59.75% & 6.09% respectively, the differences being statistically significant, too (t = 8.91) . (3) In
67.61% of alveolar hydatidosis responded to ABC-ELISA with HHF antigen in litres of 1:6400, and 74.61 % with GAH antigen, the differences between them was not statistically significant. It is suggested that the homologous antigens were both appropriate to the use in the diagnosis of alveolar hydatidosis. (4) In patienis with multiloculai echinococcosis, the geometric mean titres with GAH was 1.8577.39, it was 1.14 times higher than with HHF (l: 7555,04) , hut in patients with unilocular hydatids, the geometric mean titres with HHF (1:2891.39) is 2.31 times higher than with GAH (1: 1252.15) . It is suggested that the homologous antigen is more appropriate to the use in the diagnosis of unilocular and multilocular hydatidosis . (5) In patients with multilocular hydatidosis, a considerably higher geometric mean ritres was obtained than mothose with utiloculat hydatidosis, in the use of HHF antigens, the former is 2.61 times higher than that of the latter,and with GAH antigens, the former is 6.85 times higher than that of the latter.
出处
《地方病通报》
1989年第2期38-43,共6页
Endemic Diseases Bulletin
关键词
泡球蚴病
抗原
免疫诊断
alveolar hydatidosjs—antigen—GAH (gerbil alveolar hydatid)—HHF (human hydatid fluid)—ABC-ELISA—immunodiagosis
