摘要
目的:从噬菌体7肽库中筛选泡球蚴Em18抗原模拟表位,为研究和开发新的包虫病诊断抗原提供实验依据。方法:用纯化后的rEm18-GST免疫新西兰白兔,获得抗rEm18-GST的多克隆抗体,进一步纯化,获得抗Em18多克隆抗体IgG,以之作为靶分子,免疫筛选噬菌体随机7肽库。经过5轮的淘筛过程,随机挑取9个蓝色噬菌斑扩增,核苷酸序列测定分析并与Em18进行同源性比较。结果:经5轮筛选后,阳性克隆得到富集,9个噬菌体克隆的氨基酸序列与Em18无同源性。结论:所得肽段可能是Em18抗原模拟表位。
Objective： To screen special mimic epitopes of Echinococcus multilocularis antigen Era18 from 7 peptide library for researching and exploring new diagnostic antigens of Echinococcus multilocularis. Methods： The New-Zealand White Rabbits were immunized with the purified recombinant rEm18-GST . After obtaining the purified polyclonal antibody against rEm18-GST, we use the specific anti-Era18 IgG to screen the phage 7-mer peptide library. After 5 rounds panning, 9 clones were picked out randomly to proliferate. The proliferated clones were sequenced and their amino acid sequences were compared with Era18. Results： After 5 rounds selected and the amino acid sequences of 9 clones showed no homology with Era18. Conclusion： The peptide may be the mimic epitopes of antigen Era18.
出处
《新疆医科大学学报》
CAS
2007年第4期343-345,共3页
Journal of Xinjiang Medical University
基金
国家自然科学基金(No.30360097)
新疆维吾尔自治区高校科研计划创新研究群体基金(XJEDU2004G10)
新疆重点实验室开放课题基金资助项目(XJDX0202-2003-01)
