摘要
为对松材线虫病进行生物防治,利用海洋微生物资源,对采自青岛近海域的海水、海泥、海藻和海洋动物样品进行了细菌分离,共得到14株细菌,并采用浸渍法对这些菌株进行杀线活性的测定,从中筛选出对松材线虫具有较强杀线活性的2株海洋细菌PX3-1和PX3-2,用它们的培养滤液处理松材线虫8h,实验结果表明,松材线虫的校正死亡率分别达89.1%和91.6%。通过形态特征观察、生理生化特征测定、16SrDNA序列及其系统发育分析,鉴定菌株PX3-1和PX3-1同属于巨大芽孢杆菌(Bacillus Megaterium)。此二菌株对松材线虫具有较强的杀线活性,该研究为海洋微生物资源的利用及对松材线虫病的防治提供了生物材料和理论基础。
In order to probe the feasibility of the resources of marine microorganisms for the biological con- trol of pine wilt disease caused by the pine wood nematode, Bursaphelenchus Xylophilus （Steiner et Buhr- er） Nickle, fourteen marine bacterial strains were isolated from the samples of sea water, sea bed mud, seaweed and marine animal collected from the Yellow Sea near Qingdao, Shandong and their cultural fil- trates were assayed in vitro for nematicidal activity against B. Xylophilus using immersion test. It was found that the cultural filtrates of strain PX3 - 1 and strain PX3 - 2 displayed stronger nematicidal activity to the tested nematodes and the revised mortalities of B. Xylophilus treated for 8h were 89.1% and 91. 6%, respectively. Based on the observation of morphology, the determination of physiological and bio- chemical characteristics, the analysis of 16S rDNA sequence and phylogenetic tree, PX3 - 1 and PX3 - 2 were identified as the strains of Bacillus Megaterium. This study provides biological materials and theoreti- cal basis for the resource utilization of marine microorganisms and the control of pine wilt disease.
出处
《青岛大学学报（工程技术版）》
CAS
2012年第1期1-8,共8页
Journal of Qingdao University(Engineering & Technology Edition)
基金
国家自然科学基金资助项目(31070575)
山东省自然科学基金资助项目(ZR2010CM009)
关键词
海洋细菌
鉴定
杀线活性
松材线虫
marine bacteria
identification
nematicidal activity
Bursaphelenchus Xylophilus
