摘要
应用马来丝虫和牛丝虫成虫冰冻切片抗原作间接免疫荧光抗体试验(IFAT)和免疫酶染色试验(IEST)检测家兔经3种抗原免疫后不同时期血清抗体IgG水平,均能显示免疫动物特异性抗体水平的动态变化。IgG水平高峰(GMRT为76.11～861.08)均在动物免疫后第4wk,在第8wk时,均下降至低水平(GMRT为4.00～32.00);加强免疫后1wk时,又上升至高水平(GMRT为20.11～181.02)。
The indirect immunoflouresent antibody test (IFAT) and immunoenzymatic staining technique (IEST) with frozen sections of Brugia malayi and Setaria cervi adult worms as antigens were used to detect IgG levels in rabbits immunized with three different filarial antigens. The results showed the dynamics of the specific IgG antibody in the course of immunization. The peaks (GMRT 76.11-861.08) of feG levels in immunized rabbits appeared at the 4th week after primary immunization, which returned to (4.00-32.0 up to the 8th week after paimary immunization and rose to(20.11-181.02)at the 1st week after second immunization. It is suggested that IF AT and IEST, especially the latter, can be applied for serodiagnosis of filarial infection and both antigens are considered to be of similar diagnostic effect.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1990年第3期210-213,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
丝虫
抗原
抗体
免疫
Filaria, antigen, antibody, immune
