摘要
目的:筛选一种适合S-腺苷甲硫氨酸合成酶固定化的树脂载体,进行固定化工艺优化及固定化酶性质研究。方法:以固定化率和表观酶活回收率为指标,筛选固定化效果最佳的一种树脂,采用单因素实验对固定化条件进行优化。结果:阴离子交换树脂载体ESR-2表现出最优的固定化率(94.03%)和酶活回收率(47.45%);最佳固定化条件为加酶量4U/g、pH 8.0、15℃吸附10h,最佳条件下固定化酶表观酶活为2.1U/g,表观酶活回收率达51.6%。固定化酶的最适pH为8.5,最适温度为35℃,连续反应10批次后酶活剩余77.92%。结论:树脂载体ESR-2固定化S-腺苷甲硫氨酸合成酶酶活及稳定性较好,能够用于S-腺苷甲硫氨酸的工业化大规模生产。
Objective：The resin carrier of immobilized S-adenosyl-L-methionine synthetase was selected,and the optimal conditions of immobilization were studied.Method：The resin carrier was selected with enzyme loading and apparent activity recovery as main indexes,and optimization of immobilization conditions were determined by monofactorial experiments.Result：Anion resin ESR-2 performed well at enzyme loading（94.03%） and activity recovery（47.45%）.The optimal immobilization conditions were 4.0U/g,pH8.0,15℃ and adsorption time of 10h.Activity of immobilized enzyme was 2.1U/g,and the activity recovery was 51.6% at optimal conditions.The optimal reaction pH of immobilized enzyme was 8.5,and the optimal reaction temperature was 35℃.The residual activity was 77.92% after 10 cycles.Conclusion：The immobilized enzyme on resin ESR-2 performed well at activity and stability,and could be used in large industrial production of S-adenosyl-L-methionine.
出处
《生物技术》
CAS
CSCD
北大核心
2012年第1期90-93,共4页
Biotechnology
基金
国家重大新药创制专项项目(2009ZX09491-006)资助
