摘要
用聚苯乙烯阴离子交换树脂作载体，戊二醛为交联剂，采用吸附交联法固定α＿淀粉酶，研究了固定化条件和固定化酶的性能．结果表明，最佳固定化条件为：酶浓度１２ｇ／Ｌ，吸附温度６～９℃，吸附时间２０ｈ，戊二醛浓度４％，交联时间８ｈ，交联温度６～９℃，ｐＨ７．２．固定化酶的热稳定性、贮存稳定性、ｐＨ稳定性均比自由酶提高．最适作用温度５０℃，最适作用ｐＨ７．２，且适宜作用温度及ｐＨ均较自由酶宽，批式及连续操作实验显示出较好的操作稳定性．吸附交联联用法所得固定化α＿淀粉酶稳定性较好，克服了吸附法所得固定化酶稳定性差的缺点．
The absorbing and crosslinking method for the immobilization of α-amylase and the properties of the immobilized α-amylase were investigated. Polystyrene anion-exchange resin as carrier and the glutaraldehyde as crosslinking agent were used to prepare the immobilized α_amylase. The optimum conditions for the immobilization were as follows: 12 g/L α_amylase was immobilized on polystyrene anion_exchange resin for 20 h in 6 ℃ and pH 7.2, then crosslinked with 4% glutaraldehyde for 8h in 6℃. The stability of the immobilized α_amylase to temperature and pH has some increments. The optimum pH value of the immobilized α_amylase is 7.2 and the optimum temperature is 50 ℃. The K m value for the immobilized enzyme is higher than that of the free enzyme. The operation stability and the storage stability of the immobilized enzyme were also studied.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1998年第8期63-67,共5页
Journal of South China University of Technology(Natural Science Edition)
