摘要
以栽培表现较好杂交一代品种‘Apollo’为试材,研究了芦笋花药培养技术,建立了一套较完善的芦笋花药培养技术体系。结果表明:1～2mm的花蕾、4℃低温预处理5d、MS+2.0mg/L 6-BA+1.0mg/L NAA为愈伤组织和芽诱导的最佳条件;最适继代增殖条件为:MS+0.5mg/L 6-BA+0.2mg/L NAA。并对继代增殖组培苗进行了初步的倍性鉴定。
In order to establish a better system of Asparagus anther culture technology, a plantof the first filial generation ‘Apollo’ which had a better character in the process of growth was selected. The results showed that the best callus and shoots inducted conditions were budswhose lengths were 1-2 mm, storage at 4 ℃ for 5 days and a MS culture medium containing2.0 mg/L 6-BA, 1.0 mg/L NAA; the best subculture medium was MS＋0.5 mg/L 6-BA＋0.2 mg/L NAA. Plant regenerations from callus were ploidy identified.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2012年第1期41-45,共5页
Journal of Hebei Agricultural University
基金
河北农业大学科学发展计划(2007020)
关键词
芦笋
花药培养
技术体系
Asparagus
anther culture
technology system
