摘要
通过比较11种大孔吸附树脂对胡芦巴黄酮类化合物的静态吸附与解吸性能,筛选出DM130型大孔吸附树脂用于分离纯化胡芦巴种子中的黄酮类化合物。采用单因素方法分析该树脂富集纯化胡芦巴总黄酮的适宜工艺条件,确定优化的工艺条件为:上样量为3.64 mg黄酮/g树脂,上样液pH值5.0,吸附时间2 h,体积分数70%乙醇洗脱,洗脱速率2 mL/min,洗脱体积为150 mL,总黄酮回收率为85.05%,提取物中黄酮含量由7.8%提高到26.5%。
Eleven types of macroporous resins were applied to study static adsorption and desorption performances towards TrigoneUa foenum-graecum L. seeds flavonoids, and DM130 was the selected for the separation and purifica- tion of the crude flavonoids from Trigonella foenum-graecum L. prepared with ultrasonic-assisted extraction. Single factor experiments showed that the optimum purification procedure of total flavonoids from Trigonella foenum-graecum L. were determined as follows ： sample loading amount 3.64 mg total flavonoids/g macroporous adsorption resin at pH 5.0, adsorption duration 2 h, flow rate 2.0 mL/min, 70% ethanol ,desorption volume 3BV. Under the above opti- mized purification process, the recovery of flavonoids was 85.05% and the purity increased from 7.8% to 26.5%.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第2期224-228,共5页
Food and Fermentation Industries
基金
中国科学院生命科学领域优秀青年科技专项(KSCX2-EW-Q-23)
关键词
胡芦巴
大孔吸附树脂
总黄酮
富集纯化
Trigonella foenum-graecum L. , macroporous adsorption resin, total flavonoids, enrichment and purification
