摘要
我国啤酒大麦主要依赖进口,建立快速准确的进口啤酒大麦品系鉴定方法对保护我国啤酒制造业利益,维护国际贸易公平具有重要意义。该实验采用RAPD和2100毛细管芯片电泳技术,建立了我国主要进口啤酒大麦品系鉴定方法。采用毛细管芯片电泳技术对11次重复实验数据进行统计分析,根据谱带重现性、特异性筛选标志带,根据标志带精确长度和相对量确定判别标准。5条随机引物产生的7条标志带将19个啤酒大麦品种分成13组。
In China malting barley is mainly dependent on import. It is necessary to establish fast and accurate method for cuhivar identification to protect the benefit of brewery industry. In this paper RAPD and 2100 capillary vessel chip electrophoresis technique was applied to differentiate malting barley cultivars which were mainly used in brewery. Data of 11 repeated assays was statistically analyzed. The marker bands were selected according to reproducibility and specificity. Parameters of length and quantity of marker band were set. Seven marker bands produced by five random primers divided 19 malting barleys into 13 groups.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第2期174-179,共6页
Food and Fermentation Industries
基金
国家863计划(2011AA1008047)
国家质检总局科技计划项目(2009IK316)
关键词
啤酒大麦
RAPD
毛细管芯片电泳
品系鉴定
brewer＇ s barley, RAPD, capillary chip electrophoresis, strain identification
